[KD Validated] NDUFA13/GRIM19 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS7576-50, BS7576-100

Swiss-Prot: Q9P0J0

Host: Rabbit

[KD Validated] NDUFA13/GRIM19 Polyclonal Antibody-BS7576

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB,

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain. The protein is required for complex I assembly and electron transfer activity. The protein binds the signal transducers and activators of transcription 3 (STAT3) transcription factor, and can function as a tumor suppressor. The human protein purified from mitochondria migrates at approximately 16 kDa. Transcripts originating from an upstream promoter and capable of expressing a protein with a longer N-terminus have been found, but their biological validity has not been determined.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 17KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of variousLysates, using NDUFA13/GRIM19 Rabbit pAb antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s., Western blot analysis of extracts from wild type and NDUFA13/GRIM19 Rabbit pAb knockdown 293T cells, using NDUFA13/GRIM19 Rabbit pAb antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

Alternative Name: NDUFA13; B16.6; CDA016; CGI-39; GRIM-19; GRIM19

Immunogen: Recombinant fusion protein of human NDUFA13/GRIM19(NP_057049.5).

Modification: Unmodified

Conjugate: Unconjugated