A-RAF Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90082-50, BS90082-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P10398(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/ threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: A-RAF polyclonal antibody detects endogenous levels of A-RAF protein.

Bioworld Molecular Weight: 68 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of A-RAF on Hela cells lysates using anti-A-RAF antibody at 1/1,000 dilution., ICC staining A-RAF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: ARAF, A raf 1, A Raf proto oncogene serine/threonine protein kinase, ARAF 1, Araf, ARaf proto oncogene serine/threonine protein kinase, ARAF, ARAF1, Oncogene Araf1, Oncogene PKS2, PKS 2, PKS, PKS2, Proto oncogene Pks, Proto-oncogene A-Raf, Proto-oncogene A-Raf-1, Proto-oncogene Pks, RAFA 1, RAFA1, Ras binding protein DA Raf, Serine/threonine-protein kinase A-Raf, v raf murine sarcoma 3611 viral oncogene homolog, v raf murine sarcoma 3611 viral oncogene homolog 1, v raf oncogene homolog 1 (murine sarcoma 3611 virus),

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified