AKAP8 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS5602-50, BS5602-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O43823

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC ICC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
ICC: 1:50~1:200

Background: The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is thought to be mediated by the compartmentalization of the kinase to specific sites within the cell. To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins. The family of RII-anchoring proteins has been designated A-kinase anchoring proteins (AKAP). AKAP 95, also known as AKAP 8, is a nuclear matrix protein predominantly expressed in liver, heart, pancreas, kidney and skeletal muscle. During mitosis, AKAP 95 is recruited to the chromosomes and plays an essential role in mitotic progression. Characteristic of its family, AKAP 95 participates in PKA signaling through an interaction with the RII regulatory subunit. In addition, AKAP 95 forms a complex with HA95 and HDAC3 and is required for the deacetylation of Histone H3 in mitosis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: AKAP8 polyclonal antibody detects endogenous levels of AKAP8 protein.

Bioworld Molecular Weight: ~ 95 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of AKAP8 polyclonal antibody at 1:500 dilution
Lane 1: HepG2 cell lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: Rat kidney tissue lysate, Immunohistochemistry (IHC) analyzes of AKAP8 pAb in paraffin-embedded human tonsil carcinoma tissue at 1:5000. nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of AKAP8 pAb in paraffin-embedded human kidney carcinoma tissue at 1:5000. nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: A-kinase anchor protein 8; AKAP-8; A-kinase anchor protein 95 kDa; AKAP 95; AKAP 8; AKAP95; AKAP-95;

Immunogen: Synthetic peptide, corresponding to amino acids 373-418 of Human AKAP8.

Conjugate: Unconjugated

Modification: Unmodified