RPA1 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-64035-60, E-AB-64035-120, E-AB-64035-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: RPA1

Target Synonym: RPA1; HSSB; MST075; REPA1; RF-A; RP-A; RPA70

Research Areas: Cancer, Epigenetics and Nuclear Signaling

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human

Application: WB, IHC

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: P27694

Background: This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human RPA1 (NP_002936.1).

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000 IHC 1:50-1:100

Calculated MW: 68kDa

Observed MW: 70kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only