B3GNT4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76526-50, BS76526-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9C0J1

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase protein family. The encoded enzyme is involved in the biosynthesis of poly-N-acetyllactosamine chains and prefers lacto-N-neotetraose as a substrate. It is a type II transmembrane protein.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 42kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using B3GNT4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s., Immunofluorescence analysis of C6 cells using B3GNT4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of HeLa cells using B3GNT4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of L929 cells using B3GNT4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: B3GNT4; B3GN-T4

Immunogen: Recombinant fusion protein of human B3GNT4(NP_110392.1).

Conjugate: Unconjugated

Modification: Unmodified