HP1 alpha Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB12334-50, MB12334-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P45973

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB,ICC/IF,IHC-F,IHC-P

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Background: Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of HP1 alpha in Jurkat, C6 and 3T3Lysates using HP1 alpha antibody.

Bioworld Molecular Weight: Calculated MW: 22 kDa; Observed MW: 26 kDa

Specificity: IgG2b

Alternative Name: CBX5; HP1A; Chromobox protein homolog 5; Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha

Immunogen: Purified recombinant human HP1 alpha protein fragments expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified