SHOX Polyclonal Antibody

Catalogue Numbers: BS60246-50, BS60246-100

Sizes: 50µl, 100µl

Alternative Name: Short stature homeobox protein; Pseudoautosomal homeobox-containing osteogenic protein; Short stature homeobox-containing protein; SHOX; PHOG

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O15266

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Homeodomain proteins (HP) are transcriptional regulators that coordinate the expression of genes involved in development, differentiation and cellular transformation. HPs are characterized by a conserved domain of 60 amino acid residues that recognize and bind a site in the regulatory region of the target gene. SHOX, a member of the bicoid subfamily of the paired homeobox family, controls fundamental aspects of growth and development and undergoes splicing resulting in two isoforms, SHOXA and SHOXB. SHOXA is expressed in skeletal muscle, placental, pancreas, heart and bone marrow fibroblast and, to a lesser extent, in kidney and lung. SHOXB is highly expressed in osteogenic cells, but not expressed in brain, kidney, liver or lung. Defects in the gene SHOXX cause Leri-Weill dyschondrosteosis (LWD) and Langer mesomelic dysplasia (LMD).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SHOX polyclonal antibody detects endogenous levels of SHOX protein.

Bioworld Molecular Weight: ~ 29 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SHOX polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: sp2/0 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human SHOX.

Conjugate: Unconjugated

Modification: Unmodified