Phospho-TRIM33-S1119 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74218-50, BS74218-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UPN9

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:200 - 1:2000

Background: The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HeLa cells, using Phospho-TRIM33-S1119 antibody at 1:1000 dilution. Hela cells were treated by TGF-β at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Bioworld Molecular Weight: 150KDa

Specificity: Phosphorylated

Alternative Name: TRIM33; ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA; TIFGAMMA

Immunogen: A synthetic phosphorylated peptide around S1119 of human TRIM33(NP_056990.3).

Conjugate: Unconjugated

Modification: Phosphorylated