HSP27 (phospho-S15) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: AP0245-50, AP0245-100

Swiss-Prot: P04792

Host: Rabbit

HSP27 (phospho-S15) Polyclonal Antibody-AP0245

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IP: 1:10~1:100

Background: Hsp27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. This protein is involved in stress resistance and actin organization. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen-responsive nature of Hsp27, this protein has been studied extensively in human estrogen-responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: HSP27 (phospho-S15) polyclonal antibody detects endogenous levels of HSP27 protein only when phosphorylated at Ser15

Bioworld Molecular Weight: ~ 27 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of HSP27 (phospho-S15) pAb at 1:500 dilution
Lane 1: L02 whole cell lysate
Lane 2: SGC7901 whole cell lysate
Lane 3: HEK293T transfected with pCDNA3.1-Flag-HSP27 whole cell lysate
Lane 4: HEK293T whole cell lysate, Immunoprecipitation of L02 whole cell lysate using HSP27 (phospho-S15) pAb (Sepharose Bead Conjugate) #BD0047 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0047 (lane 4) .Lane 1 is 20% input. The western blot was probed using HSP27 (phospho-S15) pAb #AP0245.“↑”（supernatant）; “↓”（deposition）

Alternative Name: Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSPB1; HSP27; HSP28

Immunogen: Synthetic phosphopeptide derived from human HSP27 around the phosphorylation site of Serine 15.

Modification: Phosphorylation

Conjugate: Unconjugated