BRD3 (F645) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2064-50, BS2064-100

Sizes: 50µl, 100µl

Swiss-Prot: Q15059

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

Background: The bromodomain-containing proteins include BRD2, BRD3, BRD4 and BRDT. BRD2 (RING3 protein) is a mitogen-activated nuclear protein whose gene is located in the human MHC II region, suggesting its relation to HLA-associated diseases. The gene encoding BRD3 (RING3-like protein) contains two bromodomains and maps to chromosome 9q34. BRD4 (HUNK1 protein) is a nuclear protein involved in the regulation of chromosomal dynamics during mitosis. The testis-specific bromodomain protein BRDT contains a PEST sequence, indicating that it undergoes rapid intracellular degradation. The bromodomaincontaining proteins are ubiquitously expressed.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: BRD3 (F645) polyclonal antibody detects endogenous levels of BRD3 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of BRD3 pAb at 1:1000 dilution
Lane 1: SGC7901 whole cell lysate (40ug)
Lane 2: HEK293T whole cell lysate (40ug)
Lane 3: Jurkat whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of BRD3 (F645) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~72 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Bromodomain-containing protein 3; RING3-like protein; BRD3; KIAA0043; RING3L

Immunogen: Synthetic peptide, corresponding to amino acids 614-667 of Human BRD3.

Conjugate: Unconjugated

Modification: Unmodified