DDDDK-Tag (binds to flag sequnence) rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES1078-50, ES1078-100

Citations, Manuals and MSDS Available upon request.

Background: The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.

Alternate Name: DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag

Source: Rabbit

Applications: WB; ELISA; IP; IF

Dilution: Western Blot: 1/1000 - 1/3000. ELISA: 1:5000-20000. Not yet tested in other applications.

Reactivity: Species independent

Immunogen: DDDDK synthetic peptide conjugated to KLH.

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Research Use Only