MBP-Tag Monoclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-20013-60, E-AB-20013-120, E-AB-20013-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: MBP-Tag

Target Synonym: maltose-binding protein; MBP

Research Areas: Tags and Cell Markers

Conjugation: Unconjugated

Host: Mouse

Application: WB

Isotype: IgG

Clonality: Monoclonal

Clone No.: Clone:8K2

Background: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site.

Concentration: 1 mg/mL

Immunogen: Recombinant Protein

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Purification Method: Protein A purification

Dilution: WB 1:5000-1:10000

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only