CD366 Monoclonal Antibody

Catalogue Numbers: MB67160-50, MB67160-100

Sizes: 50µl, 100µl

Product: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: Q8TDQ0

Host: Mouse

Reactivity: Human

Applications: WB, FC

All Applications: WB (1/500 - 1/2000), FC (1/10 - 1/50)

Background: T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-3 is an inhibitory molecule that is induced following T cell activation. TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer, and tumor-infiltrating T cells that coexpress PD-1 and TIM-3 exhibit the most severe exhausted phenotype. Tumor-infiltrating dendritic cells (DCs) also express TIM-3. TIM-3 expression on DCs was found to suppress innate immunity by reducing the immunogenicity of nucleic acids released by dying tumor cells. Research studies show that heterodimerization of TIM-3 with CEACAM-1 is critical for the inhibitory function of TIM-3, and co-blockade of TIM-3 and CEACAM-1 enhanced anti-tumor responses in a mouse model of colorectal cancer. In addition, blockade of TIM-3 in mouse models of autoimmunity enhanced the severity of disease. Finally, binding of Galectin-9 to TIM-3 expressed by Th1 cells induces T cell death.

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of CD366 protein.

Extra Notes: Western blot analysis of CD366 expression in HepG2 (A), Daudi (B), human kidney (C) whole cell lysates.

Bioworld Molecular Weight: ~ 36-70 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: TIM3; TIMD3; Hepatitis A virus cellular receptor 2; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD-3; T-cell immunoglobulin mucin receptor 3; TIM-3; T-cell membrane protein 3

Immunogen: Recombinant fusion protein of human CD366. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified