COP1 Polyclonal Antibody

Catalogue Numbers: BS62360-50, BS62360-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8NHY2

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB:1:500~1:1000

Background: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: COP1 polyclonal antibody detects endogenous levels of COP1 protein.

Extra Notes: Western blot (WB) analysis of COP1 polyclonal antibody at 1:500 dilution
LaneA:The Testis tissue lysate of Mouse
LaneB:The Testis tissue lysate of Rat
LaneC:Hela whole cell lysate

Bioworld Molecular Weight: ~ 80 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: COP1; RNF200

Immunogen: Synthetic peptide, corresponding to Human COP1.

Conjugate: Unconjugated

Modification: Unmodified