Phospho-BRAF (Thr401) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB10424-50, MB10424-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P15056

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB,IP

All Applications: WB: 1/500-1/1000 IP: 1/20

Background: Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-BRAF in K562, C6, 3T3, HelaLysates using Phospho-BRAF antibody.

Bioworld Molecular Weight: Calculated MW: 84 kDa; Observed MW: 84 kDa

Specificity: IgG

Alternative Name: BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1

Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Thr401 of human B Raf

Conjugate: Unconjugated

Modification: Phosphorylated