TIGAR Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS78008-50, BS78008-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NQ88

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 30kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using TIGAR antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunofluorescence analysis of NIH/3T3 cells using TIGAR Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining., Immunofluorescence analysis of PC-12 cells using TIGAR Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining., Immunofluorescence analysis of U2OS cells using TIGAR Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.

Alternative Name: FR2BP; C12orf5

Immunogen: A synthetic peptide of human TIGAR(NP_065108.1).

Conjugate: Unconjugated

Modification: Unmodified