CCP110 Polyclonal Antibody

Catalogue Numbers: BS61151-50, BS61151-100

Sizes: 50µl, 100µl

Alternative Name: Centriolar coiled-coil protein of 110 kDa; Centrosomal protein of 110 kDa; CP110; Cep110; CCP110; CEP110; CP110; KIAA0419

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O43303

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Centrosome duplication and separation are linked inextricably to certain cell cycle events, specifically, activation of cyclin-dependent kinases of cyclin-dependent kinases. CP110 (centrosomal protein of 110 kDa) is a 991 amino acid cell cycle-dependent CDK substrate that regulates centrosome duplication. Localizing to the centrosome, CP110 contains ten putative CDK2 phosphorylation sites, two cyclin-binding domains and two degradation motifs. CP110 is highly expressed in testis with much lower expression in all other tissues. CP110 interacts with Ca2+-binding proteins including calmodulin (CaM) and centrin, to regulate genome stability and progression through cytokinesis. During the formation of cylindrical centrioles, it is suggested that CP110 acts as a distal end-capping protein thereby limiting the elongation of newly formed centrioles. Existing as two alternatively spliced isoforms, CP110 is observed at highest levels during the S phase of the cell cycle. CP110 becomes phosphorylated by Cdks (cyclin-dependent kinases) and is encoded by a gene located on human chromosome 16p12.3.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CCP110 polyclonal antibody detects endogenous levels of CCP110 protein.

Bioworld Molecular Weight: ~ 130 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Centriolin (R899) pAb at 1:1000 dilution
Lane 1: PC3 whole cell lysate(40ug)
Lane 2: HCT116 whole cell lysate(40ug)
Lane 3: K562 whole cell lysate(40ug)

Immunogen: A synthetic peptide corresponding to residues in Human CCP110.

Conjugate: Unconjugated

Modification: Unmodified