RPE65 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91180-50, BS91180-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q16518(Human) Q91ZQ5(Mouse) O70276(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IP

All Applications: WB:1:1,000-1:2,000
IP:1:10-1:50

Background: The retinal pigment epithelium (RPE) is a monolayer simple epithelium in proximity to the outer surface of the retinal photoreceptor cells. Retinal pigment epithelium-specific protein (RPE65) is a 65kDa protein belonging to the beta-carotene dioxygenase family. This protein is important in 11-cis retinal production as well as in visual pigment regeneration. RPE65 is attached to the membrane by a lipid anchor when palmitoylated (membrane form) and soluble when unpalmitoylated. The soluble form of the protein binds vitamin A. Defects in RPE65 causes autosomal dominant retinitis pigmentosa and/or Leber congenital amaurosis type 2.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of RPE65 on mouse eyeball cellsLysates using anti-RPE65 antibody at 1/500 dilution.

Bioworld Molecular Weight: 61 kDa

Specificity: RPE65 polyclonal antibody detects endogenous levels of RPE65 protein.

Alternative Name: RPE65, All-trans-retinyl-palmitate hydrolase, LCA 2, LCA2, Leber congenital amaurosis, mRPE 65, mRPE65, p63, rd 12, rd12, Retinal pigment epithelium specific 61 kDa protein, Retinal pigment epithelium specific 65 kDa protein, Retinal pigment epithelium specific protein, Retinal pigment epithelium specific protein 65kDa, Retinal pigment epithelium-specific 65 kDa protein, Retinitis pigmentosa 20, Retinoid isomerohydrolase, Retinol isomerase, RP 20, RP20, RPE 65, RPE65, RPE65, sRPE 65, sRPE65,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified