CD34 (L257) Polyclonal Antibody

Catalogue Numbers: BS3515-50, BS3515-100

Sizes: 50µl, 100µl

Alternative Name: CD34;
Cd34;
CD34 antigen;
CD34 molecule;
CD34;
Cluster designation 34;
Hematopoietic progenitor cell antigen CD34;
HPCA1;
Mucosialin;
OTTHUMP00000034733;
OTTHUMP00000034734;

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P28906

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:2000

Background: CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed on the surface of lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult nervous tissue. CD34 antigen expression is highest in the most primitive stem cells and is gradually lost as lineage committed progenitors differentiate. The CD34 antigen is also present on capillary endothelial cells and on bone marrow stromal cells. The CD34 cytoplasmic domain has an intracellular domain that contains consensus sites for activated protein kinase C (PKC) phosphorylation as well as serine, threonine and tyrosine phosphorylation consensus sites.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD34 (L257) polyclonal antibody detects endogenous levels of CD34 protein.

Bioworld Molecular Weight: ~ 41 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD34 (L257) pAb at 1:2000 dilution
Lane 1: The Kidney tissue lysate of Mouse(40ug)
Lane 2: The Kidney tissue lysate of Rat(40ug)
Lane 3: HEK293T whole cell lysate(40ug)
Lane 4: HepG2 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 225-270 of Human CD34.

Conjugate: Unconjugated

Modification: Unmodified