Presenilin 1 Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66379-50, MB66379-100

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Swiss-Prot: P49768

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC, IP,

All Applications: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)

Background: Presenilin 1 and presenilin 2 are transmembrane proteins belonging to the presenilin family. Mutation of presenilin genes has been linked to early onset of Alzheimer disease, probably due to presenilin's associated γ-secretase activity for amyloid-β protein processing . Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (34 kDa) and carboxy-terminal (~20, 22, 23 kDa) fragments (CTF) .

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Presenilin 1 protein.

Bioworld Molecular Weight: ~ 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Presenilin 1 expression in Hela (A), C6 (B), Jurkat (C), mouse colon (D), mouse gastric (E) whole cell lysates., Immunofluorescent analysis of Presenilin 1 staining in SKOV3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Alternative Name: AD3; PS1; PSNL1; Presenilin-1; PS-1; Protein S182

Immunogen: A synthetic peptide of human Presenilin 1

Conjugate: Unconjugated

Modification: Unmodified