Recombinant Mouse Tie2/CD202b Protein (His Tag)(Active)

Size: 200μg

Catalogue Number: PKSM040370-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: Tie2; CD202b

Target Symptom: AA517024; Cd202b; Hyk; STK1; Tie-2; Tie2

Target Species: Mouse

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-His

UNIProt ID: Q02858

Accession: Q02858

Background: TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.

Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse TEK-His at 10 μg/ml (100 μl/well) can bind human Ang2-Fc with a linear range of 6. 25-200 ng/ml.

Sequence: Met1-Lys744

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 82.4 kDa

Observed MW: 91 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only