KANK2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS67474-50, BS67474-100

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: Q63ZY3

Host: Rabbit

Reactivity: Human

Applications: WB, IHC, IF/ICC

All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)

Background: Involved in transcription regulation by sequestering in the cytoplasm nuclear receptor coactivators such as NCOA1, NCOA2 and NCOA3 .Involved in regulation of caspase-independent apoptosis by sequestering the proapoptotic factor AIFM1 in mitochondria .Pro-apoptotic stimuli can induce its proteasomal degradation allowing the translocation of AIFM1 to the nucleus to induce apoptosis .Involved in the negative control of vitamin D receptor signaling pathway.
Involved in actin stress fibers formation through its interaction with ARHGDIA and the regulation of the Rho signaling pathway .May thereby play a role in cell adhesion and migration, regulating for instance podocytes migration during development of the kidney .Through the Rho signaling pathway may also regulate cell proliferation .

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of KANK2 protein.

Bioworld Molecular Weight: ~ 120 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of KANK2 expression in A375 (A), SKOVCAR3 (B) whole cell lysates., Immunohistochemical analysis of KANK2 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of KANK2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Alternative Name: ANKRD25; KIAA1518; MXRA3; SIP; KN motif and ankyrin repeat domain-containing protein 2; Ankyrin repeat domain-containing protein 25; Matrix-remodeling-associated protein 3; SRC-1-interacting protein; SIP; SRC-interacting protein; SRC1-interacting protein

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human KANK2. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified