MMP-2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90870-50, BS90870-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P08253

Host: Rabbit

Reactivity: Human, Mouse, rat

Applications: WB, ICC IHC FC

All Applications: WB:1:1,000
ICC:1:200
IHC:1:200
FC:1:100

Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Activation of MMP-2 requires proteolytic processing. A complex of membrane type 1 MMP (MT1-MMP/MMP14) and tissue inhibitor of metalloproteinase 2 recruits pro-MMP 2 from the extracellular milieu to the cell surface. Activation then requires an active molecule of MT1-MMP and auto catalytic cleavage. Clustering of integrin chains promotes activation of MMP-2. Mutations in the MMP2 gene are associated with Torg-Winchester syndrome, multicentric osteolysis, arthritis syndrome, and possibly keloids.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: MMP-2 polyclonal antibody detects endogenous levels of MMP-2 protein.

Bioworld Molecular Weight: 74 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of MMP2 on PC12 cell lysates using anti-MMP2 antibody at 1/500 dilution., ICC staining MMP2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: MMP-2, 72 kDa gelatinase, 72kD type IV collagenase, CLG 4, CLG 4A, CLG4, CLG4A, Collagenase Type 4 alpha, Collagenase type IV A, Gelatinase A, Gelatinase alpha, Gelatinase neutrophil, Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase, Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), Matrix Metalloproteinase 2, Matrix metalloproteinase II, Matrix metalloproteinase-2, MMP 2, MMP II, MMP-2, MMP2, MMP2, MONA, Neutrophil gelatinase, PEX, TBE 1, TBE-1,

Immunogen: peptide

Conjugate: Unconjugated

Modification: Unmodified