CD63 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66137-50, MB66137-100

Product: Mouse IgG2a. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Swiss-Prot: P08962

Host: Mouse

Reactivity: Human

Applications: IHC

All Applications: IHC (1/100 - 1/300)

Background: CD63 belongs to the tetraspanin family, which is characterized by four transmembrane domains, one short extracellular domain (ECL1), and one long extracellular domain (ECL2) . Tetraspanins interact with a variety of cell surface proteins and intracellular signaling molecules in specialized tetraspanin enriched microdomains (TEMs) where they mediate a range of processes including adhesion, motility, membrane organization, and signal transduction . CD63, like other tetraspanins, is enriched in exosomes . It is also a component of Weibel-Palade bodies found in endothelial cells . Research studies demonstrate several functions of CD63 in different cell types including roles in mast cell degranulation, VEGF signaling in endothelial cells, recruitment of leukocytes to endothelial cells, and endosomal sorting during melanogenesis .

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemical analysis of CD63 staining in human malignant melanoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6. 0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 25 kDa

Specificity: Recognizes endogenous levels of CD63 protein.

Alternative Name: MLA1; TSPAN30; CD63 antigen; Granulophysin; Lysosomal-associated membrane protein 3; LAMP-3; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; Tetraspanin-30; Tspan-30; CD63

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human CD63. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified