EIF1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS60495-50, BS60495-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P41567

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: In mammalian cells, translation is controlled at the level of polypeptide chain initiation by initiation factors. Eukaryotic translation initiation factor 1 (eIF1) is crucial for the scanning process in vitro. During the scanning process, eIF1 is a component of a complex involved in recognition of the initiator codon. Translation is also initiated by the role of eIF1 in regulating the activity of ribosomal subunits 43S, 48S and 40S. eIF1 enables 43S ribosomal complexes to discern between cognate and near-cognate initiation codons, sensing the nucleotide content of initiation codons. It is also a promotor, along with eukar-yotic translation initiation factor 1A (eIF1A), for assembly of 48S ribosomal complexes at the initiation codon of a conventional capped mRNA. In addition, eIF1 and eIF1A, together with eukaryotic translation initiation factor 5 (eIF5), function in the formation of stable 40S ribosomal preinitiation complexes.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of EIF1 pAb at 1:500 dilution
Lane 1:A549 whole cell lysate (40ug)
Lane 2:Raw264. 7 whole cell lysate (40ug)
Lane 3:U-87MG whole cell lysate (40ug)
Lane 4:THP-1 whole cell lysate (40ug)
Lane 5:PC3 whole cell lysate (40ug)
Lane 6:The lung tissue lysate of Rat (40ug)
Lane 7:The spleen tissue lysate of Mouse (40ug)

Bioworld Molecular Weight: ~ 13 kDa

Specificity: EIF1 polyclonal antibody detects endogenous levels of EIF1 protein.

Alternative Name: Eukaryotic translation initiation factor 1; eIF1; A121; Protein translation factor SUI1 homolog
Sui1iso1; EIF1; SUI1

Immunogen: A synthetic peptide corresponding to residues in Human EIF1.

Conjugate: Unconjugated

Modification: Unmodified