SEC61A1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74642-50, BS74642-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P61619

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: The protein encoded by this gene belongs to the SECY/SEC61- alpha family. It appears to play a crucial role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. This protein found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 52kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of A-549 cells, using SEC61A1 Antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s., Immunofluorescence analysis of HeLa cells using SEC61A1 Rabbit pAb at dilution of 1:350. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH/3T3 cells using SEC61A1 Rabbit pAb at dilution of 1:350. Blue: DAPI for nuclear staining., Immunofluorescence analysis of PC-12 cells using SEC61A1 Rabbit pAb at dilution of 1:350. Blue: DAPI for nuclear staining.

Alternative Name: SEC61A1; HNFJ4; HSEC61; SEC61; SEC61A

Immunogen: Recombinant fusion protein of human SEC61A1(NP_037468.1).

Conjugate: Unconjugated

Modification: Unmodified