p27 Kip 1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11351-50, MB11351-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P46527

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20

Background: The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 22 kDa; Observed MW: 27 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of p27 KIP 1 in 3T3, Hela lysates using p27 Kip 1 antibody., Immunohistochemistry analysis of paraffin-embedded Human breast cancer using p27 KIP 1 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Alternative Name: CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1

Immunogen: A synthetic peptide of human p27 KIP 1

Conjugate: Unconjugated

Modification: Unmodified