HDAC4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90620-50, BS90620-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P56524(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF

All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500

Background: In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, p300/CBP, PCAF (p300/CBPassociated factor), HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: HDAC4 polyclonal antibody detects endogenous levels of HDAC4 protein.

Bioworld Molecular Weight: 120 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of HDAC4 on different lysates using anti-HDAC4 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2, ICC staining HDAC4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: HD4, HDAC4, AHO3, BDMR, EC 3.5.1.98, HA6116, HD 4, HD4, HDAC 4, HDAC A, HDAC4, HDAC4, HDACA, Histone deacetylase 4, Histone Deacetylase A, KIAA0288,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified