Matriptase (K21) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2574-50, BS2574-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9Y5Y6

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF, IP,

All Applications: WB: 1:500~1:1000
IHC/IF: 1:50~1:200
IP: 1:10~1:100

Background: Matriptase (also known as MT-SP1, ST14, prostamin and epithin in mouse) is a tumor-associated type II transmembrane serine protease that is highly expressed in many human cancer-derived cell lines and is implicated in extracellular matrix re-modeling, tumor growth, and metastasis. Matriptase performs pleiotropic functions in the development of the epidermis, hair follicles, and cellular immune system. Sphingosine 1-phosphate (S1P, SPP), present in serum-derived lipoproteins, activates matriptase while matriptase activates both urokinase-type plasminogen activator and hepatocyte growth factor (HGF). Hepatocyte growth factor activator inhibitor type 1 (HAI-1) is a Kunitz-type serine protease inhibitor identified as a strong inhibitor of matriptase and HGF.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Matriptase (K21) polyclonal antibody detects endogenous levels of Matriptase protein.

Bioworld Molecular Weight: ~ 90 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Matriptase (K21) pAb at 1:500 dilution
Lane 1: C6 whole cell lysate (40ug)
Lane 2: CT26 whole cell lysate (40ug)
Lane 3: HCT116 whole cell lysate (40ug)
Lane 4: PC3 whole cell lysate (40ug), Immunoprecipitation of HEK293T cell lysate using Matriptase (K21) pAb (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3). Lane 1 is 30% input. The western blot was probed using Matriptase (K21) #BS2574. “↑”（supernatant); “↓(deposition）

Alternative Name: Suppressor of tumorigenicity 14 protein; Matriptase; Membrane-type serine protease 1; MT-SP1; Prostamin; Serine protease 14; Serine protease TADG-15; Tumor-associated differentially-expressed gene 15 protein; ST14; PRSS14; SNC19; TADG15

Immunogen: Synthetic peptide, corresponding to the N-terminual of Human Matriptase.

Conjugate: Unconjugated

Modification: Unmodified