Human Anti-p62 (Ubiquitin Binding Protein P62 Antibody) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0521-48, ELK0521-96, ELK0521-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: anti-p62; anyi-SQSTM1

Assay Type: Sandwich

Sensitivity: 0.049 ng/mL

Standard: 8 ng/mL

Detection Range: 0.13-8 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction; Developmental science;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-p62. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-p62. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-p62, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-p62 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only