Stat3 Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9907M-50, BS9907M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P40763

Host: Rabbit

Reactivity: Human, Mouse, Rat, Zebra fish

Applications: WB, ICC/IF, IHC

All Applications: WB: 1:1000
IHC/ICC/IF: 1:50-1:200

Background: Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of Stat3 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 88 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Stat3 Rabbit mAb at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: SHG-44 whole cell lysate
Lane 3: SW480 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Stat3 antibody. Counter stained with hematoxylin., ICC staining Stat3 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Signal transducer and activator of transcription 3; Acute-phase response factor; STAT-3; APRF

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified