FOSB (Phospho-S27) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS64527-50, BS64527-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P53539

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IF

All Applications: WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200

Background: Fos and Jun dimerize to form Activator Protein-1 (AP-1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13-acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the ‘leucine-zipper’ motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP-1 dependent genes.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: FOSB (Phospho-S27) polyclonal antibody detects endogenous levels of FOSB protein only when phosphorylated at Ser27.

Bioworld Molecular Weight: ~ 36 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of FOSB (Phospho-S27) polyclonal antibody at 1:500 dilution
Lane A: Hela whole cell lysate
Lane B: U-87MG whole cell lysate
Lane C: PC3 whole cell lysate

Alternative Name: Protein fosB; G0/G1 switch regulatory protein 3; FOSB; G0S3

Immunogen: Synthetic phosphopeptide derived from human FOSB around the phosphorylation site of Serine 27.

Conjugate: Unconjugated

Modification: Phosphorylation