Recombinant Human ITGA5 Protein (His Tag)(Active)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH032600-10, PKSH032600-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: ITGA5

Target Symptom: Integrin Alpha-5; CD49 Antigen-Like Family Member E; Fibronectin Receptor Subunit Alpha; Integrin Alpha-F; VLA-5; CD49e; ITGA5; FNRA

Research Area: Signal Transduction; Stem cells

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-6His

UNIProt ID: P08648

Accession: P08648

Background: Integrin α-5 belongs to the Integrin α chain family and contains 7 FG-GAP repeats. Integrin α-5 joins with Integrin-β1 to form a fibronectin and laminin receptor which recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection; the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. It is expressed on fibroblasts; endothelial cells; peripheral T cells and platelets. Integrin α-5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains. In addition to adhesion; ITGA5 participates in cell-surface mediated signalling.

Activity: Immobilized Human NovoNectin(Cat: PKSH032450) at 2μg/ml(100 μl/well) can bind Human ITGA5-His. The ED50 of Human ITGA5-His is 18.9 ug/ml.

Sequence: Phe42-Tyr995

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 105.1 kDa

Observed MW: 130 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only