TERT Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91329-50, BS91329-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O14746(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, FC

All Applications: WB:1:1,000-1:2,000
FC:1:50-1:100

Background: Telomerase is an RNA-dependent DNA polymerase that catalyzes the addition of telomeric repeat sequences to chromosome ends. In most human somatic cells, telomerase activity is undetectable, and telomeres shorten with successive cell divisions. However, telomerase activity is detectable in immortal cells and in many human tumors. Two candidate mammalian telomerase proteins have been cloned. Human TP1 (for telomerase-associated protein 1), also designated TLP1 in rat (for telomerase protein component 1), is homologous to the Tetrahymena p80 telomerase protein and has been shown to interact with mammalian telomerase RNA. Human TERT (for telomerase reverse transcriptase), also designated hEST2 (for ever shorter telomeres), is homologous to the p123 telomerase protein from Euplotes and to the yeast Est2 protein. Expression of TERT mRNA has been shown to correlate with telomerase activity in various cell lines.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: TERT polyclonal antibody detects endogenous levels of TERT protein.

Bioworld Molecular Weight: 127/120/90/89 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of TERT on Hela cells lysates using anti-TERT antibody at 1/1,000 dilution., Flow cytometric analysis of Hela cells with TERT antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Alternative Name: TP2, TERT, CMM9, DKCA2, DKCB4, EST2, HEST2, htert, hTRT, PFBMFT1, TCS1, Telomerase associated protein 2, Telomerase catalytic subunit, Telomerase reverse transcriptase, Telomerase-associated protein 2, Telomere Reverse Transcriptase, TERT, TERT, TP2, TRT,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified