Dog NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0199-48, ELK0199-96, ELK0199-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: NT-Pro-BNP; N-BNP; ANFB_HUMAN; BNP (1-32); BNP (5-29); BNP-32; Gamma-brain natriuretic peptide; nppb

Assay Type: Sandwich

Sensitivity: 24.8 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Endocrinology; Cardiovascular biology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only