XPA Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6766-50, BS6766-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P23025

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IF

All Applications: WB 1:500 - 1:2000
IF 1:50 - 1:100

Background: The XPA gene encodes a 31 kDa zinc metalloprotein that preferentially binds to DNA damaged by UV radiation and chemical carcinogens and is required for the incision step during nucleotide excision repair. The XPB and XPD genes encode DNA helicases involved in several DNA metabolic pathways, including DNA repair and transcription, and the XPG gene product is an endonuclease that cuts on the 3' side of a DNA lesion during nucleotide excision repair. Molecular defects in the XP variant (XPV) group maintain normal excision repair, yet they result in a substantial reduction in the ability to synthesize intact daughter DNA strands during DNA replication following DNA damage.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: XPA polyclonal antibody detects endogenous levels of XPA protein.

Bioworld Molecular Weight: ~ 37 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of XPA polyclonal antibody, Western blot analysis of extracts from normal (control) and XPA knockout (KO) HeLa cells, using XPA antibody., Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using XPA antibody.

Alternative Name: DNA repair protein complementing XP-A cells; Xeroderma pigmentosum group A-complementing protein; XPA; XPAC

Immunogen: Recombinant full length Human XPA.

Conjugate: Unconjugated

Modification: Unmodified