Total Iron Binding Capacity (TIBC) Colorimetric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K071-M-48, E-BC-K071-M-96

Citations, Manuals and MSDS Available upon request.

Target Synonym: TIBC

Research Areas: Inorganic Ions

Result Type: Quantitative

Detection Instrument: Microplate reader

Tested Sample Types: Serum

Assay Time: 20 min

Detection Type: Colorimetric method

Sensitivity: 0.14 mg/L

Detection Range: 0.31-50 mg/L

Test Principle: The excess iron is added to the serum to bind all the ferritin in the serum, and the excess iron is adsorbed by adding the iron adsorbent. The iron bind with the ferritin is separated from the protein by the action of acid solution and reductant. Fe3+ in serum is reduced to Fe2+, Fe2+ binds with bipyridine to form pink complex. In a certain range, the amount of TIBC is positively correlated with the depth of color. The iron content measured is, minus serum iron value, which is called unsaturated iron binding force. Total iron binding capacity minus serum iron value is unsaturated iron binding capacity (UIBC) .

Inter CV: 2.3

Intra CV: 1.5

Storage: This product can be stored at 2~8°C for 12 months with shading light.

Shipping: Ice bag