PBR Polyclonal Antibody

Catalogue Numbers: BS5840-50, BS5840-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P30536

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:2000

Background: Mitochondrial peripheral-type benzodiazepine receptor (PBR) is an indispensable element of the steroidogenic machinery, where it mediates the delivery of cholesterol to the inner mitochondrial side chain cleavage cytochrome P-450 upon ligand activation. PBR is composed of three subunits, an isoquinoline binding site, a voltage-dependent anion channel and an adenine nucleotide carrier. PBR is genetically conserved from bacteria to humans and in humans is widely expressed in peripheral organs, whereas in the brain, it is sparse and located mainly in glial cells. Peroxisome proliferator perfluordecanoic acid (PFDA) inhibits the Leydig cell steroidogenesis by affecting PBR mRNA stability, thus inhibiting PBR expression, cholesterol transport into the mitochondria and subsequent steroid formation. A cytoplasmic protein, PRAX-1 ( peripheral benzodiazepine receptor-associated protein 1), is found to specifically interact with PBR.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PBR polyclonal antibody detects endogenous levels of PBR protein.

Extra Notes: Western blot (WB) analysis of PBR pAb at 1:2000 dilution
Lane1:PMVEC whole cell lysate(40ug)
Lane2:HepG2 whole cell lysate(40ug)
Lane3:3T3-L1 whole cell lysate(40ug)
Lane4:A2780 whole cell lysate(40ug)
Lane5:A549 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 18, 30, 32 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Translocator protein; Mitochondrial benzodiazepine receptor; PKBS; Peripheral-type benzodiazepine receptor; TSPO; BZRP; MBR

Immunogen: Synthetic peptide, corresponding to amino acids 124-169 of Human PBR.

Conjugate: Unconjugated

Modification: Unmodified