TAL-1(Phospho-Ser122)Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65228-50, BS65228-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Swiss-Prot: P17542

Host: Rabbit

Reactivity: Human

Applications: WB,ELISA

All Applications: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

Background: alternative products:The splicing pattern is cell-lineage dependent,disease:A chromosomal aberration involving TAL1 may be a cause of some T-cell acute lymphoblastic leukemias (T-ALL). Translocation t(1;14)(p32;q11) with T-cell receptor alpha chain (TCRA) genes.,domain:The helix-loop-helix domain is necessary and sufficient for the interaction with DRG1.,function:Implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of erythroid differentiation.,PTM:Phosphorylated on serine residues. Phosphorylation of Ser-122 is strongly stimulated by hypoxia.,PTM:Ubiquitinated; subsequent to hypoxia-dependent phosphorylation of Ser-122, ubiquitination targets the protein for rapid degradation via the ubiquitin system. This process may be characteristic for microvascular endothelial cells, since it could not be observed in large vessel endothelial cells.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein. Forms heterodimers with TCF3. Binds to the LIM domain containing protein LMO2 and to DRG1. Can assemble in a complex with LDB1 and LMO2. Component of a TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3.,tissue specificity:Leukemic stem cell.,

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Acetyl-TAL1/2 (K221/K222/K36/K37) Polyclonal Antibody detects endogenous levels of TAL1/2 around the acetylation site of K221 protein.

Bioworld Molecular Weight: ~ 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of MOUSE-HEART MOUSE-BRAIN using Acetyl-TAL1/2 (K221/K222/K36/K37) antibody. Antibody was diluted at 1:500.Secondary antibody(catalog#：RS0002) was diluted at 1:20000

Alternative Name: TAL1; BHLHA17; SCL; TCL5; T-cell acute lymphocytic leukemia protein 1; TAL-1; Class A basic helix-loop-helix protein 17; bHLHa17; Stem cell protein; T-cell leukemia/lymphoma protein 5; TAL2; BHLHA19; T-cell acute lymphocytic leukemia protein 2; TAL-2; Class A basic helix-loop-helix protein 19; bHLHa19

Immunogen: Synthesized acetyl-peptide derived from human TAL1/2 around the acetylation site of K221.

Conjugate: Unconjugated

Modification: Phosphorylation