PML (P39) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1296-50, BS1296-100

Sizes: 50µl, 100µl

Swiss-Prot: P29590

Host: Rabbit

Reactivity: Human

Applications: WB, IHC, IF

Background: The PML protein is a zinc finger transcription factor expressed as three major transcription products due to alternative splicing. The gene encoding human PML maps to chromosome 15q22. The t (15;17) (q22;q11.2-q12) chromosomal trans-location of the retinoic acid receptor α (RARα) gene occurs in virtually all cases of acute promyelocytic leukemia and results in the expression of a PML/RARα chimeric protein. Myeloid precursor cells expressing the PML/ RARα chimera fail to differentiate and exhibit an increased growth rate consequent to diminished apoptosis. PML/RARα transforms myeloid precursors by recruiting the nuclear co-repressor (N-CoR)-histone deacetylase complex that is essential to retinoic acid-dependent myeloid differentiation. PML/ RARα also recruits DNA methyltransferases in order to induce gene hypermethylation and silencing, which ultimately facilitates leukemogenesis.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PML (P39) polyclonal antibody detects endogenous levels of PML protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PML (P39) pAb at 1:500 dilution
Lane 1: L02 whole cell lysate (40ug)
Lane 2: HEK293T whole cell lysate (40ug)
Lane 3: A549 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of PML (P39) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 98 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Protein PML; Promyelocytic leukemia protein; RING finger protein 71; Tripartite motif-containing protein 19; MYL; PP8675; RNF71; TRIM19

Immunogen: Synthetic peptide, corresponding to the N-terminal of Human PML.

Conjugate: Unconjugated

Modification: Unmodified