CD55 Monoclonal Antibody

Catalogue Numbers: MB65794-50, MB65794-100

Sizes: 50µl, 100µl

Product: Mouse IgG1. Liquid in PBS, pH 7.3, and 0.02% sodium azide.

Swiss-Prot: P08174

Host: Mouse

Reactivity: Human

Applications: IF, FC

All Applications: IF (1/50 - 1/200)

Background: Decay-accelerating factor (DAF/CD55) is a GPI-linked plasma membrane glycoprotein normally expressed on the surface of vascular endothelial and hematopoietic cells, which are continuously exposed to autologous complement components. In conjunction with other membrane complement regulatory proteins (CD35, CD46, and CD59), DAF/CD55 protects healthy cells from inappropriate complement-mediated lysis. DAF/CD55 inhibits activation of the complement cascade by promoting membrane dissociation and inactivation of C3 convertase, which inhibits amplification of the classical and alternative complement cascades. Research studies have demonstrated that DAF/CD55 is overexpressed in a variety of solid and liquid tumors, which functions to protect tumor cells from complement-mediated attack. Given its ability to disable the complement cascade and facilitate immune evasion by tumor cells, DAF/CD55 has received attention as a potential therapeutic target for the treatment of human malignancies. CD55 deficiency is also linked to human disease. The inability to express CD55 on the surface of erythrocytes renders them highly susceptible to complement-mediated lysis, which contributes to the development of paroxymal noctural hemoglobinuria (PNH). PNH is characterized by hemolytic anaemia, pancytopenia, and venous thrombosis.

Purification and Purity: The monoclonal antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes human CD55

Extra Notes: Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD55 Antibody, followed by anti-mouse IgG PE.

Bioworld Molecular Weight: ~ 126 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: CR; DAF; Complement decay-accelerating factor; CD antigen CD55

Immunogen: Native purified human CD55.

Conjugate: Unconjugated

Modification: Unmodified