AR-α2A (R361) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2254-50, BS2254-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P08913

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC, IF

All Applications: IHC: 1:50~1:200
IF: 1:50~1:200

Background: This study investigates the involvement of α2-adrenergic receptors (AR) in mouse brain induced by a low dose of methamphetamine (METH, 2 mg/kg). Immunohistochemical studies show that α2A-AR increased in the dentate gyrus area of the hippocampus 24 h after five repeated administrations of METH. The hippocampal α2A-AR proteins rose 3.2-fold when compared to the saline-administered mice. The other adrenergic receptor, α1D-AR, were not changed by the treatment. Moreover, αo-subunits of GTP-binding proteins (Gαo), one of the downstream molecules of α2A-AR, was also increased by the treatment. These suggest that the repeated administration of low-doses of METH causes quantitative changes of the signaling of α2A-AR in the mouse hippocampus.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of AR-α2A (R361) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50. showing Nuclear staining. Negative control (the right) Using PBS instead of primary antibody,Secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 48, 54 kDa

Specificity: AR α2A (R361) polyclonal antibody detects endogenous levels of AR α2A protein.

Alternative Name: Alpha-2A adrenergic receptor; Alpha-2 adrenergic receptor subtype C10; Alpha-2A adrenoreceptor; Alpha-2A adrenoceptor; Alpha-2AAR; ADRA2A; ADRA2R; ADRAR; α-2A adrenoceptor; α-2AAR; ARα-2A; AR α2A; ARα2A; ARα 2A;

Immunogen: Synthetic peptide, corresponding to amino acids 331-380 of Human AR-α2A.

Conjugate: Unconjugated

Modification: Unmodified