PRPF19 Polyclonal Antibody

Catalogue Numbers: BS2689-50, BS2689-100

Sizes: 50µl, 100µl

Alternative Name: Pre-mRNA-processing factor 19; Nuclear matrix protein 200; PRP19/PSO4 homolog; hPso4; RING-type E3 ubiquitin transferase PRP19; Senescence evasion factor; PRPF19I; NMP200; PRP19; SNEV

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UMS4

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The spliceosome, the 0gigantic molecular machine that performs pre-mRNA splicing in eukaryotes, contains over 200 different proteins and five RNA molecules (U1, U2, U4, U5 and U6). Pre-mRNA splicing is essential to remove internal non-coding regions of pre-mRNA (introns) and to join the remaining segments (exons) into mRNA before translation. The PRP19-associated complex is required for stable association of U5 and U6 with the spliceosome after U4 is released. Changes within the spliceosome upon binding of the PRP19-associated complex include remodeling of the U6/5‘ splice site interaction and destabilization of Lsm proteins to allow further interaction of U6 with the intron sequence.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PRPF19 polyclonal antibody detects endogenous levels of PRPF19 protein.

Bioworld Molecular Weight: ~ 55 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PRPF19 polyclonal antibody at 1:500 dilution
Lane 1: The Brain tissue lysate of Rat(20ug)
Lane 2: The Brain tissue lysate of Mouse(20ug)
Lane 3: MCF-7 whole cell lysate(10ug)
Lane 4: Hela whole cell lysate(10ug)

Immunogen: Synthetic peptide, corresponding Human PRPF19.

Conjugate: Unconjugated

Modification: Unmodified