Recombinant Mouse SCF/c-Kit Ligand Protein (Active)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSM041151-10, PKSM041151-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: SCF; c-Kit Ligand

Target Symptom: FPH2; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCF; Stem cell factor; SFc-Kit ligand; SLF; steel factor; Hematopoietic growth factor KL

Target Species: Mouse

Expression Host: E.coli

Application: Cell culture

Fusion Tag: None

UNIProt ID: P20826

Accession: P20826

Background: Mouse stem cell factor (SCF), is the ligand for the receptor-type protein-tyrosine kinase KIT. It plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. It also promotes phosphorylation of PIK3R1, which is the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5.

Activity: Measured by the dose-dependent stimulation of TF-1 cells. The ED50 for this effect is 4-12 ng/mll.

Sequence: Lys26-Ala189

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 18.4 kDa

Observed MW: 16 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only