Recombinant Mouse SynCam/CADM1 Protein (Fc Tag)(Active)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSM041213-10, PKSM041213-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: SynCam; CADM1

Target Symptom: Cell adhesion molecule 1; Immunoglobulin superfamily member 4; IgSF4; Nectin-like protein 2; NECL-2; Synaptic cell adhesion molecule; SynCAM; Tumor suppressor in lung cancer 1; TSLC-1

Target Species: Mouse

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-Fc

Accession: NP_061240.3

Background: Cell adhesion molecule 1(CADM1) is a single-pass type I membrane protein and belongs to the nectin family. It contains 2 Ig-like C2-type (immunoglobulin-like) domains and 1 Ig-like V-type (immunoglobulin-like) domain. CADM1 acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. CADM1 may contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, it may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. The protein acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly. It may be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. CADM1 may play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.

Activity: Immobilized Human CADM1-Fc at 2μg/ml(100 μl/well) can bind Human CRTAMV-His(Cat: PKSH032286). The ED50 of Human SCRTAMV-His(Cat: PKSH032286) is 4. 905 ug/ml.

Sequence: Gln48-His388

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 65.3 kDa

Observed MW: 94-120 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only