Myotubularin (Y290) Polyclonal Antibody

Catalogue Numbers: BS2971-50, BS2971-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13496

Host: Rabbit

Reactivity: Human

Applications: WB IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Human MTM1, a 603 amino-acid protein, is mutated in myotubular myopathy. The largely related protein hMTMR2 is found mutated in a recessive form of Charcot-Marie-tooth neuropathy. Myotubularin is primarily a lipid phosphatase that acts on hosphatidylinositol 3-monophosphate and is involved in the regulation of the phosphatidylinositol 3-kinase (PI 3-kinase) pathway and membrane trafficking. Wildtype myotubularin can directly dephosphorylate PI 3-P and PI 4-P in vitro. Thus, it decreases PI 3-P levels by down-regulating PI 3-K activity and by facilitating the degradation of PI 3-P.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Myotubularin (Y290) polyclonal antibody detects endogenous levels of Myotubularin protein.

Extra Notes: Western blot (WB) analysis of Myotubularin (Y290) pAb at 1:2000 dilution
Lane1:SGC7901 whole cell lysate(40ug)
Lane2:HCT116 whole cell lysate(40ug)
Lane3:Panc1 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 70 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Myotubularin; MTM1; CG2

Immunogen: Synthetic peptide, corresponding to amino acids 256-3111 of Human Myotubularin.

Conjugate: Unconjugated

Modification: Unmodified