Recombinant Human Cyclophilin A Protein

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH033420-10, PKSH033420-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: Cyclophilin A

Target Symptom: Peptidyl-prolyl cis-trans isomerase A; PPIase A; Cyclophilin A; Cyclosporin A-binding protein; Rotamase A; SP18; PPIA; CYPA

Research Area: Signal Transduction; immunology;

Target Species: Human

Expression Host: E.coli

Fusion Tag: None

UNIProt ID: P62937

Accession: P62937

Background: Peptidyl-prolyl cis-trans isomerase A is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family; which catalyzes the cis-trans isomerization of proline imidic peptide bonds. Cyclophilin A regulate many biological processes; including intracellular signaling; transcription; inflammation; and apoptosis. Cyclophilin is also incorporated into many viruses; including HIV1; where it has been speculated to be involved in functions such as viral assembly and infectivity. The immunosuppressive activity of cyclosporins has been correlated with their ability to form complexes with cyclophilins that inhibit calcineurin phosphatase activity and prevent incorporation of cyclophilin into viral particles. The cyclosporin/cyclophilin complex selectively binds and inactivates calcineurin; making it a useful inhibitor for studying calcineurin activity.

Sequence: Met 1-Glu165

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of PBS, 10%glycerol, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 18 kDa

Observed MW: 16 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only