PLK1(Phospho-Thr210)Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65122-50, BS65122-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Swiss-Prot: P53350

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, IF (paraffin section), ELISA

All Applications: WB 1:500-2000 ,Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Background: polo like kinase 1(PLK1) Homo sapiens The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015],

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phospho-Plk (T210) Polyclonal Antibody detects endogenous levels of Plk protein only when phosphorylated at T210.

Bioworld Molecular Weight: ~ 70 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of 293 using p-Plk (T210) antibody., Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PLK1 (Phospho-Thr210) Antibody, Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PLK1 (Phospho-Thr210) Antibody. The picture on the right is blocked with the phospho peptide.

Alternative Name: PLK1; PLK; Serine/threonine-protein kinase PLK1; Polo-like kinase 1; PLK-1; Serine/threonine-protein kinase 13; STPK13

Immunogen: The antiserum was produced against synthesized peptide derived from human PLK1 around the phosphorylation site of Thr210. AA range:176-225

Conjugate: Unconjugated

Modification: Phosphorylation