Rad50 (A708) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2232-50, BS2232-100

Sizes: 50µl, 100µl

Swiss-Prot: Q92878

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Rad52 family members (Rad50, Rad51B/C/D, Rad52, Rad54, MRE11) mediate DNA double-strand break repair (DSBR) for DNA damage that otherwise could cause cell death, mutation or neoplastic transformation. Rad51 (RECA, BRCC5) interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of Rad51. Rad52 forms a heptameric ring that binds single-stranded DNA ends and catalyzes DNA-DNA interaction necessary for the annealing of complementary strands.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Rad50 (A708) polyclonal antibody detects endogenous levels of Rad50 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Rad50 (A708) polyclonal antibody at 1:500 dilution
Lane 1: 786-O whole cell lysate (40ug)
Lane 2: U-87MG whole cell lysate (40ug)
Lane 3: SGC-7901 whole cell lysate (40ug)
Lane 4: SK-OVCAR3 whole cell lysate (40ug)
Lane 5: The Testis tissue lysate of Mouse (40ug)
Lane 6: The Testis tissue lysate of Rat (40ug)
Lane 7: C6 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Rad50 (A708) pAb in paraffin-embedded human breast cancer tissue.

Bioworld Molecular Weight: ~ 153 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: DNA repair protein RAD50; hRAD50; RAD50

Immunogen: Synthetic peptide, corresponding to amino acids 670-722 of Human Rad50.

Conjugate: Unconjugated

Modification: Unmodified