CD45 (Phospho-Y1216) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS64566-50, BS64566-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P08575

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB:1:500~1:1000

Background: The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing. The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates. CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling. Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD45 (Phospho-Y1216) polyclonal antibody detects endogenous levels of CD45 protein only when phosphorylated at Tyr1216.

Bioworld Molecular Weight: ~ 180 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot (WB) analysis of CD45 (Phospho-Y1216) polyclonal antibody at 1:500 dilution
Lane A: HEK293T whole cell lysate
Lane B: MCF-7 whole cell lysate
Lane C: PC12 whole cell lysate
Lane D: AML-12 whole cell lysate

Alternative Name: Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; PTPRC; CD45

Immunogen: Synthetic phosphopeptide derived from human CD45 around the phosphorylation site of Tyrosine 1216.

Conjugate: Unconjugated

Modification: Phosphorylation